Clinical and Experimental Pediatrics

Original Article

Fcr Receptor and Mac-1 Expression and Functional Differentiation of HL-60 Cells by All-trans Retinoic Acid

Hae Soon Kim, Kyung Hyo Kim, Gyoung Hee Kim, Ju Young Seoh,

Clin Exp Pediatr. 1999;42(4):462-471. Published online April 15, 1999

Purpose : During differentiation of HL-60 cells by all-trans retinoic acid(ATRA), we analyzed the expression of Fcr receptors and Mac-1 by molecules of equivalent soluble fluorochromes(MESF) and functional studies. Methods : HL-60 cells were induced to differentiate by adding 1μM ATRA. On the 4th and 7th day after stimulation as well as before stimulation, the cells were analyzed for phenotypic and...

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Functional Differentiation of HL-60 Cells by Dimethylsulfoxide and Phorbol 12-myristate 13-acetate

Hae Soon Kim, Kyung Hyo Kim, Gyoung Hee Kim, Ju Young Seoh

Clin Exp Pediatr. 1999;42(3):355-363. Published online March 15, 1999

Purpose : During hematopoietic differentiation of HL-60 cells by DMSO and PMA, we demonstrated functional changes of HL-60 cells-phagocytic activity, respiratory burst, antibody-dependent cell-mediated cytotoxicity(ADCC) and opsonophagocytic activity. Methods : HL-60 cells(ATCC CCL-240), cultured in RPMI 1640 and supplemented with 10% FBS, were induced to differentiate by adding 1.0% DMSO or 16nM PMA. On the 4th and 7th day after...

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The Utility of Serum Prealbumin Concentrations for Short-term Nutritional Assessment in Patients with Acute Diarrhea

Kyoung Ah Lim, Hae Soon Kim, Jeong Wan Seo, Seung Joo Lee

Clin Exp Pediatr. 1999;42(1):88-93. Published online January 15, 1999

Purpose : To evaluate the usefulness of serum prealbumin concentration for the nutritional assessment of acute diarrhea. Methods : From February 1997 to August 1997, ninety patients with acute diarrhea(age, 4-24 months) were randomly assigned into two groups on the basis of their intakes for 2 days prior to admission : liquid diet group(N=56, clear liquid and/or oral electrolyte solutions), and...

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